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Exactly where L is column duration and N the number of theoretical plates.The much larger the amount of theoretical plates the decrease is HETP and much better would be the column efficiency.This will slow down the movement in the sample throughout the “column” and as the sample will come out another side of the column, it is actually detected.

If no anaerobic expansion of microorganisms is detected on Columbia agar, the product or service complies with the test.After completion of incubation period observe the plate below colony counter and Convey the result as colony forming unit (CFU) for every gm/ml, by multiplying regular amount of CFU/pate with dilution factor. The dilution issue is